NADH Oxidase Activity of Mitochondrial Apoptosis-inducing Factor

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NADH oxidase activity of mitochondrial apoptosis-inducing factor.

Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein, which translocates to the nucleus during apoptosis and causes chromatin condensation and large scale DNA fragmentation. Here we report the biochemical characterization of AIF's redox activity. Natural AIF purified from mitochondria and recombinant AIF purified from bacteria (AIFDelta1-120) exhibit NADH oxidase activity, whereas su...

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Role of apoptosis-inducing factor, proline dehydrogenase, and NADPH oxidase in apoptosis and oxidative stress.

Flavoproteins catalyze a variety of reactions utilizing flavin mononucleotide or flavin adenine dinucleotide as cofactors. The oxidoreductase properties of flavoenzymes implicate them in redox homeostasis, oxidative stress, and various cellular processes, including programmed cell death. Here we explore three critical flavoproteins involved in apoptosis and redox signaling, ie, apoptosis-induci...

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Apoptosis-inducing factor

Although the seminal paper by Kerr, Wyllie and Currie (Kerr et al., 1972), who coined the word ‘apoptosis’, stated that only nuclei but not cytoplasmic organelles would undergo major modifications in the dying cell, recent evidence suggests that no cellular compartment is spared (Ferri and Kroemer, 2001). Mitochondria are particularly affected early during the apoptotic process, and they are no...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2001

ISSN: 0021-9258

DOI: 10.1074/jbc.m010498200